FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14697137
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17450.017.0611461
GTTCTAG43850.016.6578161
GTATTAG28300.016.0247061
GTCCTAG37400.015.8281331
GTGCTAG35700.015.8060941
GGTCTAA26600.014.3158211
GCTCTAG47450.014.2996381
GTACTAT29050.014.0618051
GTCTTAG33500.013.6406261
GTACTAA26650.013.2497641
GTATATA54550.013.009611
GTGTTAG37200.012.8422561
GTATTAA46750.012.7365561
GGTCTAG27650.012.6453671
GCCCTAG35600.012.6414991
GTCTTAT39200.012.6285981
TTGCGCG6050.012.5471312
CCCTTAG39450.012.4608171
GTTCTAA45450.012.339181
CTATACT36400.012.330964
Produced by FastQC (version 0.11.5)