FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14249562
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17800.014.9288731
GTATAGG25550.014.8579211
GTTCTAG39500.014.5906841
GTCCTAG34700.014.3215251
TAGTGCG6850.014.31710757
GTATTAA44100.013.7730571
GTGCTAG35350.013.7653081
GTATTAG26550.013.6483771
GTACTAA28500.013.3199961
GTACAGG62800.013.1888781
GTCTTAG32200.013.1827351
CAGTACT50550.013.1079974
GTGCTAA37200.013.0807441
CTGTACT69050.013.0446694
TATACTG43400.012.9614665
GGTCTAA26750.012.901271
CTATACT33050.012.7392724
GCTCTAG41550.012.7079851
GTTCTAA43700.012.4776241
GTCCTAA31350.012.4393361
Produced by FastQC (version 0.11.5)