FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14249562
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17450.018.2169761
GTCCTAG37000.016.6227251
GTGCTAG35850.015.5174611
GGTCTAA27100.014.7901521
GTATTAA45450.014.5205621
GTCTTAG34200.014.4475381
GTATATA54050.014.0001381
GTTCTAG41750.013.9866641
GTACTAA26350.013.899821
GTATTAG30400.013.6392861
TCTGTAC57350.013.6009073
GCCTTAG33400.012.9314581
GTGTTAG36100.012.9214281
GCTCTAG39150.012.8856121
GTGCTAA35400.012.7865091
CTATACT34600.012.7671284
GGTCTAG25750.012.7476561
CCTATAC23400.012.6845623
GTACAGG60500.012.3933381
GTTCTAA41350.012.2836021
Produced by FastQC (version 0.11.5)