FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14240446
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17850.017.980911
GTATTAG29450.016.523451
GTCCTAG35500.015.8462391
GTGCTAG36300.015.7822311
GTACTAA28350.014.7298821
GTTCTAG40550.014.6387681
GTATTAA44250.014.1946621
GTCCTAA28500.014.0468861
GTATAGG22850.014.0463561
GCTCTAG41250.013.7210341
GTATAAG29650.013.385671
GGTCTAA26100.013.2228911
GTACAGG61400.012.5343981
CCTATAC24050.012.48453
GTCTTAG31800.012.4806641
CGATCGT4205.7716534E-912.32657638
GTATATG42900.012.2279391
GTCTAGA32300.012.1806171
TCTATAC29800.012.160183
TCTAGAC33050.012.113053
Produced by FastQC (version 0.11.5)