FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14240446
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28550.018.5098191
GTCCTAG37500.018.3289951
GTGCTAG36650.016.8692531
GTACTAG17000.016.0507351
GTTCTAG38750.015.95504951
GTATTAA45350.015.3847671
GTCTTAG32750.015.2923261
GTACTAA27350.015.0281821
GTATATA52000.014.0150921
GTACAGA64850.013.3684341
GTCCTAA31150.013.3057691
TCTAGAC29700.013.1312543
GTACATA41300.013.1300511
GCGTTAG5053.6379788E-1212.9950821
TCTGTAC58550.012.9682093
GTGTTAG37000.012.8823231
GCGATCG5155.456968E-1212.73228137
GGTCTAG27700.012.3444651
GTCTTAA38600.011.9904181
GGTCTAA25650.011.9844881
Produced by FastQC (version 0.11.5)