FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13040788
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14650.016.4515441
GTATTAG25250.015.5449631
GTCCTAG31550.014.8417721
CTATACT29750.014.5825944
GTATTAA38150.014.5303981
TCTATAC29250.014.360853
GTATAGG20000.014.2887521
TATACTG37750.014.2285465
GTCTTAG27400.014.0738741
GTGCTAG32200.014.0075331
GTATATA44150.013.9594581
GTTCTAG34950.013.69348051
CTAGTAC14500.013.5348373
GTGTTAG34050.013.3475961
GCTCTAG36700.013.2281541
GTACTAA24550.013.1832521
GTACAGA62100.013.1402311
GTGCTAA31150.012.71118451
GTATTAT39850.012.7009211
GGTCTAA25250.012.6814171
Produced by FastQC (version 0.11.5)