FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13040788
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15500.020.2573241
GTATTAG27950.016.1719321
GTACTAA26100.015.86401751
GTCCTAG33150.015.7168611
GTTCTAG35750.015.1529091
GTTCTAA37450.015.111
GTGCTAG34600.014.7590371
GGTCTAA24500.013.9425291
TATACCG4950.013.9107945
TACGCGA2253.5031167E-513.7896642
GTATTAA36650.013.4627711
GTGCTAA30550.013.4402741
GCTCTAG36050.013.3997041
GTATAGA28450.013.340831
GTCTTAG30400.013.166091
TACTAGG20750.013.1251042
GTGTTAG30250.013.1173131
GCCCTAG30500.012.8966651
CGATCGT5350.012.87364838
TCTAGAC28400.012.8577853
Produced by FastQC (version 0.11.5)