FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12807618
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15250.016.9343381
GTACTAA23950.016.3898931
GTATTAA36250.015.9579851
GTCCTAG29400.015.4597821
GTTCTAG37600.015.201871
GTATTAG25250.014.4551051
GTCTTAG28550.014.1109581
GTATATA45750.013.9990511
GGTCTAG25100.013.9927541
CTATACT29550.013.8663724
TATACTG37200.013.7916645
TCTAGAC28550.013.6284263
GTACATA35450.013.5984241
TCTGTAC54300.013.3799963
GTACGGA8350.013.1959371
GTTCTAA35900.013.1402281
CTAAGCG7650.013.0529894
CTAGACT25800.012.9456964
GCTCTAG37550.012.8379261
GTCTTAA33450.012.7644571
Produced by FastQC (version 0.11.5)