FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12807618
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14600.017.7031631
GTCCTAG32450.017.629331
GTATTAG23950.017.2670521
GTTCTAG38350.015.3664361
GTACTAA24750.014.898791
GTCCTAA31650.014.2639561
GTGCTAG31500.014.2224781
GTCTTAG28650.014.1938361
GTATATA45450.013.7242021
GCGATCG4851.8189894E-1213.49009437
CTATACT27050.013.365064
GTTCTAA35550.013.0868931
GGTCTAA23950.012.9502891
GTATTAA35850.012.7851231
TCTATAC27300.012.7388823
CTTAGAC24700.012.6857763
GCTCTAG37000.012.6671711
GTCTTAA33750.012.559541
GTATAGG21450.012.53169251
CGATCGT5004.0017767E-1112.39746238
Produced by FastQC (version 0.11.5)