FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12629154
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24650.017.039821
GTTCTAG35650.016.7073921
GTACTAG15050.016.0134031
GTCCTAG29650.014.7469131
GTATAGG20150.014.6941841
GTACTAA23450.014.6817981
GTATTAA36550.014.6004551
GTGCTAG30350.014.1799071
GTTCTAA34450.013.5916371
GTATAAG27200.013.4171121
GTCTTAG28550.013.3856341
TCTATAC27550.013.3716273
GTGCTAA30950.013.3488131
GTCCTAA25700.013.2624621
CTATACT28500.013.1675644
TCTAGAC28500.012.9259063
TATACTG38650.012.8273465
GTACAGG53500.012.741881
GTATATA44550.012.3650061
GTATAAA45450.012.3474081
Produced by FastQC (version 0.11.5)