FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12629154
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15800.019.6291641
GTATTAG24200.018.0844251
GTTCTAG37800.016.0449071
GTCCTAG31050.015.8705061
GTGCTAG31100.014.9585581
GTCTTAG27400.014.7147081
GTATAGG20550.014.4212531
GTTCTAA35950.014.0907741
GTATATA42850.014.0735481
GTATTAA34750.013.7840351
GTCCTAA27950.013.68541
GTGCTAA29450.013.4564011
GGTCTAG23100.013.2768291
CTATACT30500.013.210534
GGTCTAA22750.013.1781431
GCTCTAG32800.013.0275941
GTACAGG53500.012.9466861
CTAGACT28300.012.8989284
GCGATCG5205.456968E-1212.58340737
GTCCTAT26250.012.4712691
Produced by FastQC (version 0.11.5)