FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13335486
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24300.017.4007471
GTACTAG16050.017.3491841
GTACTAA22600.015.6674331
GTCCTAG31600.015.2303531
GTGCTAG32750.015.2203881
GTTCTAG36100.014.3793341
GTATATA39050.013.821261
GTCTTAG29400.013.7976061
GTCTTAA30850.013.5948271
GTATAGG19100.013.1388861
GTTCTAA31700.013.1218541
GGTCTAA23100.013.0960331
GTATAAA42000.012.9323321
GTATTAA33650.012.6679311
GTATTAT33200.012.6325441
GTCCTAA27250.012.6154441
GTACAGG57850.012.5979991
GTACAGA58950.012.5378681
GGATTAG26500.012.4535861
TATACTG34500.012.2570055
Produced by FastQC (version 0.11.5)