FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12928657
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14650.017.1336881
GTCCTAG33450.016.3443131
GCTCTAG35500.015.4973431
GTCTTAG26750.015.2963851
GTATTAG24900.014.6376771
GTTCTAG34400.014.4935631
GTGCTAG29250.014.1065571
GGTCTAA22700.014.0871361
GTACTAA20600.014.0209581
GTATAGG20350.013.6863061
GTCTTAA33050.013.6290461
TCTATAC24050.013.5824843
GTATTAA31150.013.2461241
GTCCTAA25500.013.2145241
GGTCTAG21350.013.2063131
GTTCTAA32550.013.2045821
CTATACT26350.013.179984
GTACTAT22050.013.0989451
GTATATA36500.013.0002541
GTACAGG57750.012.8607821
Produced by FastQC (version 0.11.5)