FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13239961
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17000.015.7777181
GTATAGG18550.015.7569991
GTGCTAG31300.015.7105081
GTCTTAG27450.015.5338051
GTACTAA22550.015.4018651
GTATTAA34300.015.2387021
GTATTAG22500.014.8247611
GTTCTAA33550.014.55441
GTTCTAG36500.014.4144171
TCTAGAC27700.014.40074353
GGTCTAA22450.013.7855681
GTCCTAG30800.013.7326041
GTCTTAA30250.013.6412561
GTATATA39300.013.4749361
GGTCTAG24300.013.3020961
GCGCTAG4406.366463E-1113.2860161
TCTATAC21550.013.0849883
CTATACT25900.013.0118674
TATACTG33500.012.9342335
GTATAAA45200.012.6289771
Produced by FastQC (version 0.11.5)