FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12391321
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13950.016.3157841
GTATTAG22750.014.6221421
GTCCTAG29300.014.5801171
GTATTAA32450.014.351781
GTATAGG18600.014.3076881
GTCTTAA32100.014.1810071
GTTCTAG34500.013.9049771
TCTAGAC27800.013.6012683
GTACTAA21900.013.4308631
GTGCTAG29550.013.3902831
GTATAAG22800.013.0542781
GTCTTAG25900.012.8437731
GTACATA29750.012.8294581
GTATTAT34000.012.6676181
GCTCTAG33800.012.5353781
ATCTCCG124450.012.48757370
CTATACT27000.012.4478864
CTAAGCG7600.012.437654
GTCCTAA25150.012.3914131
TCTCCGA106500.012.35743170
Produced by FastQC (version 0.11.5)