FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12391321
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG33500.015.9943651
GTACTAA21300.015.7838141
GTACTAG13550.014.7318031
GTCCTAG30350.014.5389211
GTGCTAG27350.014.2130071
GTATTAG21300.013.9752521
GTATAGA23900.013.3341051
TCTGACG101850.013.29595170
TCTATAC24250.013.2720153
GTATATA36450.013.2587291
GTATAGG18700.013.1092181
GGTCTAG25000.013.0275661
GTCTTAG27750.012.8723411
GTTCTAA32750.012.831881
GGTCTAA21700.012.749341
GTATTAA33100.012.6961951
GTCCTAA27500.012.6073231
GCTCTAG38700.012.306891
GCGCTAG5451.0913936E-1112.2089251
GTATTAT32900.011.9218181
Produced by FastQC (version 0.11.5)