FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12148784
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21800.015.8989591
GTCCTAG30000.014.5874291
GTACTAG14300.014.4446131
GTATTAA31650.014.1587931
TCTAGAC26450.013.6296683
GTATAGA24550.013.5475911
ATCTCCG115350.013.1687970
GTATTGG25500.012.76829051
GGTCTAG25200.012.6424381
GTATATA38750.012.4680171
GTTCTAG34950.012.3210551
GTTCTAA30000.012.253441
GCTCTAG33800.012.2223661
GTCCTAT23550.012.1902591
CTAGACT26450.011.9093214
GTACTAT22300.011.7746061
GTCTTAG27550.011.6911221
CGATCGT5207.7852746E-1011.44528538
GTGCTAG27950.011.3985491
GCCCTAG32000.011.3781931
Produced by FastQC (version 0.11.5)