FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12148784
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22250.016.5195831
GTACTAG12100.015.9117191
GTCCTAG29300.015.2926281
GTGCTAG29400.014.7643441
GTTCTAG32450.014.2396391
GTATTAA32800.013.8742431
GTACAGG56200.013.2673161
GTGCTAA28250.013.2588281
GTATATG31200.013.0149711
GTCCTAA25450.012.9294441
GTATAGG18750.012.8821281
GGTCTAA22950.012.6600421
GTCTTAG27850.012.56941
GTCTTAA30950.012.5545781
GTGCATA26050.012.4972651
GTATATA37050.012.4716951
GCCCTAG30400.012.4362651
TAGTACT23700.012.3971514
GTATAAA40950.012.3952091
GTGTTAG29550.012.3201391
Produced by FastQC (version 0.11.5)