FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12183454
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG28850.015.8971781
GTATTAG21950.015.6309771
GTACTAG15000.015.4044861
GTACTAA20100.014.2827661
GTCCTAA23600.014.0930871
GTATTAA31550.013.9818851
GTATAGG18350.013.9277621
GTCTTAG26800.013.4554111
GCCCTAG31250.013.3318821
GCTCTAG33450.013.1876971
GTACATA30650.012.9075121
GTATATA34950.012.8220471
GGTCTAA22500.012.7592721
GTCTTAA30350.012.6890341
GGTCTAG25150.012.66770651
CTATACT24250.012.5572664
ATCTCCG113800.012.54802170
GTGCTAG28300.012.4948061
CCCTTAA32150.012.1963991
GTGCTAA25900.011.7601811
Produced by FastQC (version 0.11.5)