FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11878300
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13700.017.6356141
GTCCTAG30250.016.321341
GTTCTAG32100.015.4897851
GGTCTAG22350.014.4135971
CTAGCGT4801.8189894E-1213.8553984
GTCTTAA28800.013.4956121
GTATTAG21800.013.4922651
GTATAGG16800.013.3392921
GTCTTAG23900.013.3323151
GTATATA35650.013.259781
GTCCTAA24700.013.1840261
CTAGACT26500.013.0766344
GTGCTAG27550.012.9640491
ATCTCCG107850.012.91560470
GTATTAA34200.012.9004991
GGTCTAA20800.012.6258331
GTACTAA18950.012.564981
GCTAGAC19100.012.4618413
TCTAGAC26100.012.204153
GTGCTAA26950.011.6935351
Produced by FastQC (version 0.11.5)