FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12038412
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG44200.015.6105731
GTACTAG21450.014.977341
GTCCTAG34150.014.2180571
GTCTTAG37200.014.0337071
GCGAGAC47700.014.0058743
GTGCTAG38600.013.8084481
CGAGACT49700.013.4421464
GTATTAG38900.013.0450121
GATCTAG30050.012.9992591
GTCCAGG85200.012.5975791
GCCCTAG28750.012.4442161
GAGCGAG56750.012.3513571
GTACTAA33650.012.1510071
AGCGAGA57350.012.0946212
GCTCTAG35600.011.7930781
GTACAGG42850.011.7572991
TCTAGAC35250.011.7029913
GCTAGAC26400.011.4776153
CAGGACT70400.011.4602354
GGTTTAG33200.011.4360111
Produced by FastQC (version 0.11.5)