FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11873179
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG21350.016.9293231
GTCTTAG36350.014.1617541
GTGCTAG36250.013.797961
CGAGACT49100.013.5296944
GTCCTAG35300.013.4453141
GTACAGG44100.013.4115431
GTATAGG24550.013.3842291
GCGAGAC50250.012.7840713
GTCTAGG31900.012.5893781
GTTCTAG44800.012.5500371
GTATTAG36650.012.4519781
GCCCTAG29650.012.4365231
GTCCAGG86450.012.3738531
GTACCGT4455.9117156E-912.3064721
GGTCTAG25200.012.1697341
GCTCTAG32200.011.9051741
CCAGGAC84400.011.7197793
GAGCGAG55650.011.4808811
GTACTGG41700.011.3817651
GTACTAA32400.011.3809551
Produced by FastQC (version 0.11.5)