FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11873179
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAG22550.015.5300671
GCGAGAC47450.015.5300553
GTACTAG20800.015.0828621
GTATTAG38300.014.6679911
CGAGACT47150.014.6236644
GTTCTAG44200.014.2781681
GTCCTAG33600.014.2226551
GCCCTAG29200.013.4924121
GTCTTAG34250.013.3136811
GGTCTAA27800.013.2533371
GTGCTAG35950.013.0899951
AGCGAGA56800.012.7163862
GTATAGG22200.012.4884711
GAGCGAG56100.012.4849561
GTACTAA31350.012.4507321
GTCCTGG91550.011.9938011
GTACAGG44450.011.9820121
GTGCTGG190950.011.901921
GTGCAGG79250.011.5537541
GTGCAGA73650.011.3425671
Produced by FastQC (version 0.11.5)