FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10497915
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC44800.016.132083
GTTCTAG36950.015.4161811
CGAGACT45000.015.2492514
GTGCTAG32100.015.2428581
GCTCTAG28050.013.9289231
GTATTAG31950.013.4858331
GAGCGAG51350.013.3685411
GCCCTAG27600.013.09763051
GGTCTAG22650.012.8969911
AGCGAGA52700.012.8827212
GTGCAGG72850.012.831491
GTCCTAG31750.012.5357241
GGTCTAA23350.012.5103581
GTACTAG19700.012.4186731
GTCTTAG31900.011.90444851
GTCCAGA48200.011.8937631
GGTCAGG124350.011.7751171
GGTCAGA51800.011.7720841
GCAAGAC78800.011.6728663
GTCCTAA26500.011.5744421
Produced by FastQC (version 0.11.5)