FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8621681
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA61550.023.268608145
CCTGTCG27600.020.269798145
GTCCTAG30750.017.1475811
GTATTAG32900.016.3831481
GTACTAG18450.015.2422941
GTATTAA48400.015.0100361
GTACTAA28400.014.8531861
GTGCTAG35700.014.6058641
GTCTTAG33200.014.2939551
TGACGCG11008.95418E-414.240454145
GCTCTAG40200.013.991061
GCGCTAG5152.1790584E-713.6514711
CGACGAA54450.013.562337145
CTAGACT28050.013.5604544
GTCCTAT30300.013.5350811
GTACTAT30800.013.5055751
GGTCTAA28750.013.4496671
CGCCGAA17300.012.935177145
GTGTTAG37700.012.8985891
GTCTTAA40550.012.8589241
Produced by FastQC (version 0.11.5)