FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8365527
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA68100.034.214005145
GGGGGGG449400.020.883892145
GTACTAG18550.017.0431651
GTTCTAG38850.016.5768531
CTATACT32500.016.3930994
GTATTAA48300.016.363911
TATACTG41550.015.4998425
GTATTAG30350.014.6607221
GTCGCTT22705.711627E-1014.389068145
GTACTAA29100.014.2845251
GTCCTAG32150.014.2041111
GTATAAG31650.014.0585421
GTGCTAG34150.014.0580021
GTATAGG22450.013.5608651
GTCCTAA29450.013.5183611
GTCTTAG31050.013.1988741
GCTCTAG39300.012.9606611
TGCACTG88300.012.730525
GTACTGG56450.012.6530821
GTATATG44900.012.6481151
Produced by FastQC (version 0.11.5)