FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13663666
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG24600.020.926541
GTATTAG43350.017.0127961
GTTCTAG49850.016.3325181
GGTCTAG30900.016.1873421
GTCCTAG41200.015.6851791
GTATTAA64500.014.7738681
GTGCTAG45300.014.7491271
GTCTTAG39600.014.5671741
GCCCTAG38900.014.3600261
CTAGACC32450.014.0629164
GCGAGAC50800.014.0136913
GTACAGG55100.013.9811951
GTCCTAA36500.013.5037491
GTGTTAG52750.013.358211
GTCTAGC28900.013.1385961
GTACTAA37650.012.994311
TGCACTG90400.012.8421795
GCTCTAG39550.012.8316271
GTGCAGG103400.012.7467681
CCTAGAC41000.012.7330913
Produced by FastQC (version 0.11.5)