FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12263218
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG22350.018.127261
GTGCTAG38650.018.037261
GTATTAG37050.016.8459171
GGTCTAG30300.016.0212271
GTACTAA36750.015.8909311
GTTCTAG44950.015.7528371
GTCCTAG38550.015.5276661
GTCTTAG37350.015.4402091
GCGAGAC49900.015.0665713
GTCCTAA33250.015.0388861
GTTCTAA49700.014.6879221
CGAGACT49800.014.4373154
CTAGACC28850.013.9154224
GTGCAGA83550.013.8483981
GTGCAGG92400.013.6280551
GCCCTAG33050.013.5837731
GTATTAA63800.013.3297481
TCTAGAC36150.013.3264163
GTGCTAA37000.013.2187321
GCGCTAG5251.8189894E-1213.2093381
Produced by FastQC (version 0.11.5)