FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13687390
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG72350.033.067905145
TCTCGTT66900.031.210283145
TGCCGTG89150.027.486958145
CACGAGC148650.024.14191145
TCGTATT42950.022.112581145
GTCCTAG54500.021.8374791
CTACATG134850.020.806133145
TCTCCGC156100.020.621078145
ATGCCGG81950.020.612862145
GTATTAG44500.020.0585821
CGTCTTA36850.019.674053145
GTACTAA40550.018.4332311
ACACATA147700.017.81794145
CTCCGAC60250.017.808855145
TACATCG71450.017.553965145
GTACTAG26900.017.2656461
GTATATA73350.017.214891
CTATACT47100.017.08564
GTTCTAA61400.016.9014031
GTGCTAG53650.016.0965331
Produced by FastQC (version 0.11.5)