FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13687390
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT22000.028.340305145
CCGTATA16400.025.6397145
TGACGCG23750.021.673223145
TTATACC92350.020.725061145
GTGCTAG54300.020.4622781
GTACTAG25000.020.3338891
CTGCCGC231850.019.824896145
GTATTAG42200.019.6180021
GTTCTAG61250.019.3260861
GTACTAA43500.019.1986621
GCGTTAG11900.018.3078211
GTCCTAG55900.018.0578231
GTATAGG33300.017.8826561
GTATAGA46750.017.5533181
GTCCTAA48350.017.4230381
GGTCTAG49350.017.36431
GTATTAA66650.017.3244461
CTATACT49500.017.1450824
GCTCTAG64200.017.0806471
GTCCTAT48000.016.7936131
Produced by FastQC (version 0.11.5)