FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13174352
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG69750.032.01427145
TCTCGTT66100.027.639881145
TGCCGTG87200.025.940311145
CGTCTTA35950.025.410238145
GTACTAG25450.022.8019391
CACGAGC139150.022.091223145
GTCCTAG55500.021.826951
GTATAGG33400.019.5463491
TCTCCGC146900.019.395807145
GTATTAG42500.019.286751
TCGTATT43250.019.109785145
CTACATG132900.018.493189145
GTACTAA39850.018.3849561
GTGCTAG56400.017.7488231
GTATTAA67700.017.5721441
ACACATA147400.017.460953145
CTCCGAC58600.017.320784145
CCCACGC109200.017.129086145
GTTCTAA61100.016.502251
ATGCCGG72300.016.345055145
Produced by FastQC (version 0.11.5)