FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13174352
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA15050.029.86578145
CGCCGTT20600.027.09831145
TGACGCG23950.023.30794145
GTACTAA40050.020.8635221
TTATACC93500.020.004457145
GTGCTAG52300.019.4499551
GTCTTAG50200.019.250421
GTTCTAG59450.017.9662631
GTCCTAG53950.017.9123461
GTACTAG24850.017.5435371
GTTCTAA61150.017.4667911
GTATTAA61400.017.3956721
GTCCTAA46600.017.30731
CTGCCGC225050.017.234293145
GATAGTT55550.017.096449145
GTAGATA78700.016.765455145
GGTCTAG40750.016.7607151
GCCGACC49900.016.562391145
GTACAGG102500.016.233191
GGTCTAA40200.016.0863041
Produced by FastQC (version 0.11.5)