FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13419530
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG83000.038.78282145
TCTCGTT71250.031.54361145
TGCCGTG102900.027.971107145
GTACTAG25950.022.9148081
CACGAGC180150.021.651226145
ACACATA160100.021.419254145
CTACATG143350.021.140396145
TCGTATT52350.020.634983145
GTGCTAG51550.020.1162531
CGTCTTA49150.019.765863145
CGTATGG43850.019.3442145
GTACTAA38900.018.6418971
GTATTAA68700.018.577861
GTATAGG35850.018.407381
GTATTAG43700.017.9218181
GTCCTAG54200.017.7947581
CTCCGAC60850.017.752527145
GTTCTAG61650.017.5264071
TCTCCGC191200.017.404411145
ATGCCGG97300.017.212091145
Produced by FastQC (version 0.11.5)