FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13180443
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT24300.034.615646145
CCGTATA21950.034.02698145
TGACGCG29650.022.500072145
GCCGACC55900.021.144457145
GTACTAA40950.020.897771
GTACTAG24300.020.891211
GTCCTAG54650.020.8344151
TTATACC130550.020.051693145
GTTCTAG57050.019.5765841
GTCCTAA44800.019.2637481
GTGCTAG51100.019.1595251
GTAGATA101650.018.832905145
GATAGTT64700.018.604778145
TCTAGAC49250.018.40483
TAGATCG20500.018.393747145
GTCTTAG52300.018.0265871
GTATATA70950.017.8878231
GTATTAG41200.017.7785361
GACGAAT39500.016.889294145
GTATTAA63450.016.8018681
Produced by FastQC (version 0.11.5)