FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16579170
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA18550.030.87497145
CGCCGTT22500.026.743546145
GTACTAG29950.022.306231
GTCCTAG67450.022.17791
TGACGCG28850.020.857183145
GTTCTAG69500.020.2699241
GTACTAA49050.020.1342331
CTGCCGC273250.019.580309145
TTATACC114900.019.05506145
GTGCTAG63600.018.6108261
GTATTAG50900.018.4037841
GTTCTAA74750.018.0691181
GTCTTAG60850.017.900531
TACGCGA6950.017.7470782
GTCCTAT58400.017.6567461
GTCCTAA56000.017.6354311
GTATTAA84450.017.0255341
GTCTTAA74250.016.9193941
GTACTAT50600.016.5037441
GTGCTAA64300.016.375411
Produced by FastQC (version 0.11.5)