FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16040375
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG75300.025.129538145
GTACTAG31400.023.5623191
TCTCGTT74150.023.172676145
AGACGGC134550.023.169878145
TGCCGTG95300.021.453354145
TCCTATG157950.021.022524145
GTATTAG51700.020.6240671
CCTATCG48300.019.963812145
CACGAGC91400.019.751139145
CTATACT57350.019.5942124
GTACTAA48600.018.5068681
TCGTATT39100.018.356817145
CTCCGAC72000.018.225737145
ACACATA183750.018.1497145
TCTCCGC151000.018.101034145
GTCCTAG61050.017.8218631
CGTCTTA35800.017.618753145
GTATTAA77100.017.498711
GTTCTAA78450.017.1051271
GTGCTAG60450.016.9188291
Produced by FastQC (version 0.11.5)