FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16013052
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG84400.025.25497145
TCTCGTT78750.022.279572145
CGTCTTA47700.021.735003145
GTATTAG49900.021.3568461
GTACTAG31600.021.1067351
TGCCGTG109050.020.54352145
CTCCGAC77050.020.136461145
AGACGGC188800.020.045206145
GTGCTAG62700.019.8875471
GTATTAA80050.019.5619751
ACGGACG49950.019.304482145
GTCCTAG68450.019.276061
GTCCTAA54650.019.1026081
CACGAGC127550.018.700682145
ACACATA204750.018.129593145
CGAATCG12850.017.4883049
ATGCCGG95950.017.454565145
TCCTATG177300.017.338005145
CTATACT55000.017.2659684
GTTCTAA72550.017.0875191
Produced by FastQC (version 0.11.5)