FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625597
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG3150.021.9091
GTCCTAG5850.014.7464421
TACGGGT1951.5963912E-414.1571254
GTGCTAA6400.014.0183371
GTTCTAG6950.013.9019711
GTATTAG3608.896677E-913.4192621
ATACGAC1550.0081017313.3579333
ATAAGCG1850.001550701113.24146756
TAGACTC6000.013.2280645
GTACAGG10500.013.1454011
GCCTTAG4801.8189894E-1112.9400031
TAGGACT5350.012.9001844
TAGTACC3508.896495E-812.8172544
GGCCGAA3255.03951E-712.7409261
ATAGGGC4902.7284841E-1112.6764053
GCCTATA4902.7284841E-1112.6759221
CTAGGCC6650.012.4540114
GTACTAA3902.7253918E-812.38701251
GTCCTAT6150.012.3438521
GTGCAGG21150.012.236411
Produced by FastQC (version 0.11.5)