FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625597
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG1004.2836522E-420.73789445
CTAAGCG1200.001471639717.26017240
TAAGCGA1704.9301372E-516.24858341
AACCGTC1450.00383568114.95247667
GCCCTAG6350.014.7098981
CCTAGAC5250.014.4736663
CTTATAC5500.014.4437623
CGTAGAC1951.5840895E-414.1700223
CGAGCGT2454.966696E-614.08027616
TCTAGAC5550.013.6913053
GTCCGGA3651.0411895E-813.2695071
GGATACG1600.0098258312.9733121
GCGAATC2153.6106326E-412.85653944
TCCGCGC2153.6216964E-412.8518813
GTGCTAA6200.012.8338161
GTGCTAG5451.8189894E-1212.6955971
GTATATG8000.012.5408691
GTACTAG2509.099937E-512.4543811
GGTCTAA4459.877112E-1012.4388311
GTACCGA1950.00256483412.384136
Produced by FastQC (version 0.11.5)