FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607621
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG3000.019.5966551
GCGCATA900.005657864719.2124081
GTCGTAA1250.001910808616.599521
GGACTAG3402.3646862E-1015.2569111
TAACGTC1605.761421E-415.09379131
CTTAACG1400.004100599314.8030723
GTGCTAG5850.014.7787731
TACTAGG4051.4551915E-1114.5065592
GGTCTAA4353.6379788E-1214.3099311
TAACGAG1450.005215808714.2764495
GTTCTAG6550.013.7273381
TCAGTAC6400.013.4923833
GGTCTAG4901.8189894E-1213.4094751
CTAGGCT7550.013.257544
ATAGGAC5155.456968E-1212.7430983
TCTAGAC5155.456968E-1212.7430983
ACTAGGG4651.5097612E-1012.627713
GTATATG6300.012.6252971
TAGGACC4651.5279511E-1012.6185054
GGTTTAG6050.012.5753931
Produced by FastQC (version 0.11.5)