FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2653572
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA5150.016.0831811
TACCGTA1300.002518381715.9273357
GTACTAA4800.015.0989251
GTACTAG3302.6102498E-914.6413811
CCCTTAA6300.014.2429761
GTCTAGT3403.976311E-914.2107531
ATTAGAC5450.013.9313793
CTAGCCT5450.013.2981354
GCCTTAG5850.012.9788071
TCTAGCC6550.012.64555553
TACTAGG3551.06903826E-712.6381342
TATACTG7950.012.589225
CCTGTAC11100.012.4366943
GTTCTAG6150.012.3456951
GCTCTAG7300.012.2918841
GTCCTAG6200.012.2461321
GTATAGG4256.877599E-912.1806451
CTAGCCC5701.8189894E-1212.1094134
GTATATA8350.011.9861431
CTAAGCT6700.011.8473434
Produced by FastQC (version 0.11.5)