FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2653572
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG750.00589445829.18022570
GGTCTAG3050.019.252131
GTCCTAG6500.019.1301731
GTACTAG3401.4551915E-1116.2543951
GTCTAGT2855.8971636E-915.7553581
CGAATCG1350.003242761115.3345949
GGCCTAG5950.013.9323391
GTATTAG5000.013.81623651
TCTAGAC5350.013.5439363
GTTCTAG5900.013.4649761
GCGAATC1800.001403602413.417778
GGACTAG3102.8045542E-713.370551
TCTAGGC7350.013.1446813
ATTAGAC5700.012.7122913
CTAGACT4104.0436134E-912.6234654
GTGCTAG5257.2759576E-1212.5004041
CTAGTCT4451.029548E-912.4059814
TCTATAC5054.7293724E-1112.2987373
CTATACC5359.094947E-1212.2538054
TTAGACC4802.6011548E-1012.2204453
Produced by FastQC (version 0.11.5)