FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594006
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA4300.016.8481541
TCTAGAC4750.014.5257283
GCTCTAG6900.014.4994131
GTTCTAG6250.014.35141851
TACTCGA1750.006394083613.84433369
CTAGACC4304.1836756E-1113.6389834
GTATTAG5350.013.5415081
GTACCGG2557.5066364E-613.5288631
GAACTAG5450.013.2930391
GTATAGG4457.2759576E-1113.1792411
GTATAGT4852.3646862E-1112.8036041
GTCTTAG5950.012.7557851
CTAGGAC5850.012.3841133
GCTAGAC3651.5429941E-712.2871733
GGTCTAG5401.0913936E-1112.1383971
GTCCTAT4853.110472E-1012.0922931
GTATAAG4601.7407729E-911.9995141
GTACTAC3205.9009726E-611.8588941
GTCTAAT4105.484435E-811.7800111
GTACAAG8250.011.7086161
Produced by FastQC (version 0.11.5)