FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10447560
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG20450.015.70628773
CGAGCCC90150.014.17054473
GTCCTAG26450.014.0764781
CCGAGCC122250.013.76373373
TCCGAGC138350.013.42835573
CTAAGCG7000.013.0365774
GTATTAG20550.012.9667331
CGATCGT4052.1109372E-812.61703238
GCGATCG3851.485023E-712.324429537
CTCCGAG175850.011.85162573
CCCTTAG26950.011.7836541
CTAGACT22100.011.56185154
GTGCTAG23950.011.4307641
GCTCTAG28150.011.2813321
ACGAGAC28250.011.24048372
GGTCTAG18850.011.2314581
GTCTTAG22850.011.1823061
TCTCCGA187400.011.10169972
GTATAGG18250.011.0006751
GTCCTAA22600.010.9829751
Produced by FastQC (version 0.11.5)