FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10198422
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112310.1101248801040004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA23500.018.32841573
GTCCTAG23850.016.2255171
CCGACGA28850.014.67651572
TGACGCT127850.014.16092973
GTTCTAA30750.014.1280721
GTTCTAG27750.013.6820561
GTACTAG12300.013.6531781
GACGAAT18600.013.54088173
GACGCTG115150.013.25022173
GTCTTAG21300.013.19751551
GCCGACG34900.012.5506671
GTCCTAT21000.012.5168281
GTATTAG20300.012.4089241
GTCTATA18950.012.329681
ACGCTGC89450.012.20117473
CTGACGC150300.012.11859772
GTGCTAG25600.011.8363881
GTATAGG15750.011.8214481
TCTAGGC31700.011.6299893
GTATTAA31300.011.5470721
Produced by FastQC (version 0.11.5)