FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10618101
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG18650.015.26523473
TCCGAGC137500.013.88310873
CGAGCCC90250.013.83141673
CCGAGCC120950.012.73486373
GTATTAG21650.012.6450851
GTGCTAG25200.012.4570831
GTCCTAG24800.012.363631
GTACTAG12450.012.3139771
GTCTTAG24000.012.1673821
GCTCTAG29400.012.1673811
CTAGTAC11550.012.0092523
GTTCTAG28450.011.932161
GGATTAG21800.011.7208731
CTAAGCG7250.011.5798834
GTATAGG18300.011.5689861
ACGAGAC25200.011.44232672
CTATACT27050.011.3351254
GTGCTAA26550.011.2737331
GTCCTAT24600.011.1287041
GTACAGG46250.011.0492981
Produced by FastQC (version 0.11.5)