FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10420835
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA22000.015.26527773
GTACTAA21000.014.9661061
TGACGCT130250.014.8818373
GTGCTAG23500.014.6180571
GTCCTAG22450.014.487831
GGTCTAA19900.013.9569391
GACGCTG118250.013.42850473
GACGAAT21900.013.33476773
GTACTAG13450.013.3138451
GTCTAGA23900.012.9972281
GTATAAG20050.012.5766331
CTGACGC156200.012.315865572
GTCTTAG21700.012.2939881
GTTCTAG27650.012.2918561
GTATTAA32900.011.9965821
CCGACGA27700.011.86036872
ACGCTGC92850.011.59790873
CGCGTCA6357.2759576E-1211.49470515
GTTCTAA30600.011.4651431
GTACTAT18350.011.3518981
Produced by FastQC (version 0.11.5)