FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16359197
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20450.017.443311
GTCTTAG40800.016.976761
GTCCTAG42350.016.7643011
GTATTAG35400.016.6314721
GTTCTAG50950.016.041791
GTACTAA33700.015.3123341
GTCCTAA39700.015.0917851
GTATTAA56950.014.8990081
GTATAGG27550.014.3307281
GTTCTAA49300.014.2604791
GTGCTAG40400.014.0587771
CTATACT41850.014.0363544
GCTCTAG48800.013.9098121
GGTCTAG31250.013.6313881
GTATATA64400.013.2829981
GTGTTAG45100.013.1312051
CCTATAC27700.013.1066623
TCTATAC37300.013.0705193
GTATAAG32900.013.0530141
TATACTG51650.012.90543655
Produced by FastQC (version 0.11.5)