FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16174653
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG33850.016.2180791
GTCCTAG39950.015.9888011
GTACTAG20600.015.9227221
GTGCTAG41150.014.7673911
GTATAGG29600.014.2307911
GTACTAA35200.014.1247511
GTATTAA55100.014.0364521
GTATATA66850.013.8418571
GTGTTAG40800.013.79394151
GTATAAG33650.013.6466961
GCTCTAG46450.013.6027371
GTTCTAG48400.013.4113791
GTACAGG69400.013.2835071
GTTCTAA51800.013.0643331
GTCTTAG38450.013.0206471
TCTAGAC36700.012.9829663
CTAGACT37300.012.9595384
TCTGTAC66900.012.9025293
GTATAAA68550.012.8941721
GTCCTAA35700.012.8630621
Produced by FastQC (version 0.11.5)