FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16174653
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18850.019.6647011
GTATTAG36250.018.1576881
GTCCTAG41150.017.6792721
GTGCTAG38250.016.6648461
GTTCTAG48900.016.3650781
GTCTTAG39350.015.3186121
GTACTAA31450.013.9893651
GTATTAA55850.013.89441
GTGTTAG41850.013.5757331
GTATATA60550.013.388031
GGTCTAA31100.013.1442731
GTGCGTA8450.013.1192261
TCTAGAC37450.012.926463
GTTCTAA49100.012.9117331
GCTCTAG46000.012.8028321
GTACATG62150.012.70892051
GTCTATA33100.012.6640411
TATACTG53150.012.6048445
GTGCTAA39700.012.5657061
GTACTAT32650.012.5202711
Produced by FastQC (version 0.11.5)